Cai J, Jiang Y, Zhang Y, Lu G, Zhang X, Gao Q, Zuo L
Department of Surgery, Hebei Medical College, Shijiazhuang.
Chin Med J (Engl). 1995 Sep;108(9):669-73.
Quantitative analysis of P53 protein expression was performed on paraffin-embedded tissues from 55 smooth muscle tumors of the gastrointestinal tract, using immunofluorescence and flow cytometry. No positive expression was found in normal smooth muscle tissues of the gastrointestinal tract. Over-expression of P53 gene was found in a significantly higher proportion in leiomyosarcomas (90%) and potentially malignant smooth muscle tumors (75%) as compared to leiomyomas (14%) (P < 0.005). The quantitation of P53 expression was found to be progressively enhanced in the sequence from leiomyoma through potentially malignant smooth muscle tumor to leiomyosarcoma (P < 0.005). It was markedly over-expressed when the mitotic counts ranged from one to more than one per 10 high power fields (P < 0.005) or the mild cytologic atypia was found (P < 0.005). The five-year survival rate was significantly higher in patients with low-expression of P53 than in those with over-expression of P53 (P < 0.005). It was suggested that P53 over-expression might be associated with the transformation of leiomyoma into leiomyosarcoma and could be used as an objective parameter in distinguishing the malignant from the benign and predicting the prognosis of patients with smooth muscle tumors of the gastrointestinal tract.
采用免疫荧光和流式细胞术对55例胃肠道平滑肌肿瘤石蜡包埋组织进行P53蛋白表达的定量分析。在胃肠道正常平滑肌组织中未发现阳性表达。与平滑肌瘤（14%）相比，平滑肌肉瘤（90%）和潜在恶性平滑肌肿瘤（75%）中P53基因的过表达比例显著更高（P<0.005）。发现P53表达的定量在从平滑肌瘤到潜在恶性平滑肌肿瘤再到平滑肌肉瘤的序列中逐渐增强（P<0.005）。当有丝分裂计数为每10个高倍视野1个至超过1个（P<0.005）或发现轻度细胞异型性时（P<0.005），其明显过表达。P53低表达患者的五年生存率显著高于P53过表达患者（P<0.005）。提示P53过表达可能与平滑肌瘤向平滑肌肉瘤的转变有关，可作为鉴别胃肠道平滑肌肿瘤良恶性及预测患者预后的客观参数。
