Zhang J, Lnoue K, Nakashima N, Hiramatsu S, Ohashi M, Umeda F, Nawata H
Department of Endocrinology, China Medical University, Shenyang.
Chin Med J (Engl). 1994 Sep;107(9):699-702.
All diabetic patients should be screened for the early stage of diabetic nephropathy, because microalbuminuria has a great prognostic significance. The albusure test (AT), alatex agglutination nephelometric immunoassay, is a rapid and low cost test for the detection of microalbuminuria of 30 mg/L or more. We compared the results of AT and of radioimmunoassay (RIA) for urinary albumin to evaluate the clinical utility of AT using fresh urine samples from 74 diabetic patients without persistent proteinuria and from 11 healthy subjects. Urinary albumin levels measured by RIA were 6.0 +/- 2.3 mg/L in the healthy subjects, 11.0 +/- 8.7 mg/L in the AT-negative group (n = 61), and 38.1 +/- 10.2 mg/L in the AT-positive group (n = 13). Using a cut-off value of 30 mg/L by RIA, the rate of coincidence between AT and RIA was 89.2%, although five subjects were false-positive by AT, and three were false-negative. These results show that AT may provide a useful monitor microalbuminuria, a reliable early mark of diabetic nephropathy.
所有糖尿病患者均应接受糖尿病肾病早期筛查，因为微量白蛋白尿具有重要的预后意义。Albusure检测（AT），即乳胶凝集比浊免疫测定法，是一种用于检测30mg/L及以上微量白蛋白尿的快速且低成本的检测方法。我们比较了AT和放射免疫分析法（RIA）检测尿白蛋白的结果，以评估AT的临床实用性，使用了来自74例无持续性蛋白尿的糖尿病患者和11例健康受试者的新鲜尿液样本。通过RIA测量，健康受试者的尿白蛋白水平为6.0±2.3mg/L，AT阴性组（n = 61）为11.0±8.7mg/L，AT阳性组（n = 13）为38.1±10.2mg/L。以RIA的30mg/L临界值计算，AT与RIA的符合率为89.2%，尽管有5名受试者AT结果为假阳性，3名受试者为假阴性。这些结果表明，AT可能是监测微量白蛋白尿的有用方法，微量白蛋白尿是糖尿病肾病可靠的早期标志。
