Green G A, Riot B, Monteil H
Laboratoire de Toxinologie Bactérienne, Faculté de Médecine, Université Louis-Pasteur, Strasbourg, France.
Eur J Clin Microbiol Infect Dis. 1994 Jul;13(7):576-81. doi: 10.1007/BF01971309.
A 33 basepair oligonucleotide probe, designed from the sequence of the Clostridium difficile toxin B gene, was evaluated for its ability to detect toxigenic Clostridium difficile directly in stool samples, without culture or DNA isolation. Two different labelling techniques were investigated: radiolabelling and digoxigenin-labelling. One hundred ninety-six stools were tested, with a good correlation (96%) obtained between the oligonucleotide probe and the gold standard, the cytotoxicity tissue culture assay. The sensitivity and specificity were 83% and 100%, respectively. In parallel, a new commercially available enzyme immunoassay for the detection of Clostridium difficile toxin A in stool specimens was investigated. In 162 samples tested, a sensitivity of 80% and a specificity of 98% were obtained.
从艰难梭菌毒素B基因序列设计的一段33个碱基对的寡核苷酸探针，被评估了其在粪便样本中直接检测产毒艰难梭菌的能力，无需培养或DNA分离。研究了两种不同的标记技术：放射性标记和地高辛标记。对196份粪便进行了检测，寡核苷酸探针与金标准细胞毒性组织培养试验之间获得了良好的相关性（96%）。敏感性和特异性分别为83%和100%。同时，对一种新的用于检测粪便标本中艰难梭菌毒素A的市售酶免疫测定法进行了研究。在检测的162份样本中，敏感性为80%，特异性为98%。
