Utermöhlen O, Tárnok A, Bönig L, Lehmann-Grube F
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Germany.
Eur J Immunol. 1994 Dec;24(12):3093-9. doi: 10.1002/eji.1830241227.
Blocking the interleukin-2 receptor's alpha-chain in lymphocytic choriomeningitis virus-infected mice by treatment with monoclonal antibodies diminished the increase of numbers of CD8+ T lymphocytes in spleens and prevented CD8+ T lymphocyte-mediated virus clearance from organs as well as generation of virus-specific cytotoxic T lymphocytes. Also, the CD8+ T cell-mediated early phase of the delayed-type hypersensitivity footpad swelling reaction was decreased. The same treatment had no effect on the number of CD4+ spleen T lymphocytes, which, however, did not enlarge during infection, but these cells' heightened DNA synthesis and cytokine production were reduced by antibody treatment; yet the generation of antiviral antibodies remained unaffected, and the CD4+ T lymphocyte-mediated second part of the footpad reaction was somewhat augmented. We conclude that blocking of the interleukin-2 receptor by antibody in lymphocytic choriomeningitis virus-infected mice diminishes both CD8+ and CD4+ T cell-mediated antiviral immune responses, the former more than the latter.
通过用单克隆抗体治疗，阻断淋巴细胞性脉络丛脑膜炎病毒感染小鼠体内白细胞介素-2受体的α链，可减少脾脏中CD8⁺ T淋巴细胞数量的增加，并阻止CD8⁺ T淋巴细胞介导的病毒从器官中清除以及病毒特异性细胞毒性T淋巴细胞的产生。此外，CD8⁺ T细胞介导的迟发型超敏反应足垫肿胀反应的早期阶段也会减弱。相同的治疗对CD4⁺ 脾脏T淋巴细胞的数量没有影响，而在感染期间这些细胞数量并未增加，但抗体治疗可降低这些细胞增强的DNA合成和细胞因子产生；然而，抗病毒抗体的产生仍未受影响，并且CD4⁺ T淋巴细胞介导的足垫反应的第二部分有所增强。我们得出结论，在淋巴细胞性脉络丛脑膜炎病毒感染的小鼠中，抗体阻断白细胞介素-2受体可减弱CD8⁺ 和CD4⁺ T细胞介导的抗病毒免疫反应，前者比后者更明显。
