Schillaci R, Ribaudo C M, Rondinone C M, Roldán A
Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina.
Immunol Cell Biol. 1994 Aug;72(4):300-5. doi: 10.1038/icb.1994.45.
In a serum-free medium addition of insulin-like growth factor-1 (IGF-1) consistently enhanced lymphocyte proliferation response to PHA in a dose-dependent fashion. This effect was produced by an acceleration in the expression of clone expansion and not in the number of proliferating cells. This was documented by kinetics data obtained from the first proliferation round of PHA-stimulated lymphocytes, in which addition of IGF-1 reduced G1-phase length, without changing G0-phase, S-phase or cloned size. The data were confirmed in 10-day culture of stimulated lymphocytes where IGF-1 only accelerated cell proliferation without modifying the area enclosed by the proliferation curve. As IGF-1 is under the control of growth hormone, our results suggest that some of the immuno-regulation effects ascribed to growth hormone in vivo could be produced by IGF-1.
在无血清培养基中，添加胰岛素样生长因子-1（IGF-1）可始终如一地以剂量依赖方式增强淋巴细胞对PHA的增殖反应。这种效应是通过加速克隆扩增的表达而非增殖细胞数量产生的。这一点由PHA刺激淋巴细胞第一轮增殖的动力学数据得以证明，其中添加IGF-1可缩短G1期长度，而不改变G0期、S期或克隆大小。在刺激淋巴细胞的10天培养中证实了该数据，其中IGF-1仅加速细胞增殖，而不改变增殖曲线所包围的面积。由于IGF-1受生长激素控制，我们的结果表明，体内归因于生长激素的一些免疫调节作用可能由IGF-1产生。
