Rodríguez-Ithurralde D, Vincent O
Division of Neuromyology, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Montevideo, Uruguay.
J Neurol Sci. 1994 Jul;124 Suppl:52-3. doi: 10.1016/0022-510x(94)90176-7.
We measured cholinergic markers and acetylcholinesterase (AChE) molecular forms after glutamate receptor stimulation of superfused slices of mouse spinal cord at different developmental ages. AChE globular forms were secreted in a dose-dependent fashion. A period of selective sensitivity to excitotoxic agents was detected by increased acetylcholine (ACh) release and AChE secretion (sAChE) at postnatal day 14. Strychnine-resistant glycine stimulation potentiated glutamate-induced AChE release, suggesting N-methyl-D-aspartate (NMDA) receptor involvement.
我们在不同发育阶段对小鼠脊髓灌流切片进行谷氨酸受体刺激后，测量了胆碱能标志物和乙酰胆碱酯酶（AChE）的分子形式。AChE球状形式以剂量依赖性方式分泌。在出生后第14天，通过增加乙酰胆碱（ACh）释放和AChE分泌（sAChE）检测到对兴奋性毒性剂的一段选择性敏感时期。抗士的宁甘氨酸刺激增强了谷氨酸诱导的AChE释放，提示N-甲基-D-天冬氨酸（NMDA）受体参与其中。
