Van Camp J M, Drongowski R, Gorman R, Altabba M, Hirschl R B, Coran A G
Section of Pediatric Surgery, C.S. Mott Children's Hospital, Ann Arbor, MI 48109.
J Pediatr Surg. 1994 Oct;29(10):1348-51. doi: 10.1016/0022-3468(94)90113-9.
Seventy-four New Zealand white rabbit pups were divided into four groups: group I, 2 days of age (n = 9); group II, 3 to 5 days of age (n = 24); group III, 6 to 8 days of age (n = 27); and group IV, 10 to 13 days of age (n = 14). Mouth swabs (MS), rectal swabs (RS), small bowel specimens (SB), and large bowel specimens (LB) were obtained from each rabbit, incubated for 24 hours in thioglycolate broth, and plated on blood agar in aerobic and anaerobic environments. After 24 hours, growth on blood agar plates were observed. All MS specimens and all but one RS specimen showed positive growth. Growth of both LB and SB specimens increased significantly with age (P < .04). In addition, SB growth was significantly less than RS or MS growth in groups I, II, and III (P < .05). LB growth was significantly less than RS or MS growth in group I (P < .01) and tended to be less in groups II and III (62.5% v 100% and 93% v 100%, respectively). These data show that nearly half of normal rabbits under 6 days of age have sterile small and large intestines despite almost 100% growth from rectal and mouth swabs. These findings partially explain the absence of spontaneous bacterial translocation in young rabbit pups (under 4 days of age) and have important implications for the prophylaxis and treatment of neonatal sepsis.
74只新西兰白兔幼崽被分为四组：第一组，2日龄（n = 9）；第二组，3至5日龄（n = 24）；第三组，6至8日龄（n = 27）；第四组，10至13日龄（n = 14）。从每只兔子获取口腔拭子（MS）、直肠拭子（RS）、小肠标本（SB）和大肠标本（LB），在硫乙醇酸盐肉汤中培养24小时，然后在需氧和厌氧环境下接种于血琼脂平板上。24小时后，观察血琼脂平板上的生长情况。所有MS标本以及除一个之外的所有RS标本均显示生长阳性。LB和SB标本的生长均随年龄显著增加（P <.04）。此外，在第一、二、三组中，SB的生长显著低于RS或MS的生长（P <.05）。在第一组中，LB的生长显著低于RS或MS的生长（P <.01），在第二组和第三组中LB的生长有降低趋势（分别为62.5%对100%和93%对100%）。这些数据表明，尽管直肠拭子和口腔拭子的生长率几乎为100%，但6日龄以下的正常兔子中近一半的小肠和大肠是无菌的。这些发现部分解释了幼兔（4日龄以下）无自发性细菌移位的现象，并对新生儿败血症的预防和治疗具有重要意义。
