Honkaniemi J, Kononen J, Kainu T, Pyykönen I, Pelto-Huikko M
Department of Neurology, Department of Veterans Affairs Medical Center, San Francisco, CA 94121.
Brain Res Mol Brain Res. 1994 Sep;25(3-4):234-41. doi: 10.1016/0169-328x(94)90158-9.
We have previously demonstrated that stress causes a rapid and transient elevation in the expression of the immediate early genes (IEGs) c-fos and the members of the jun gene family in the brain. Here we demonstrate the effect of stress on the expression of fra-1, fra-2 and recently characterized IEGs encoding zinc finger containing proteins. Capsaicin-induced stress caused a rapid and transient induction of NGFI-A, NGFI-B, fra-2 and TIS11 in the hypothalamic paraventricular nucleus. The NGFI-A mRNA levels were also slightly increased in the cerebral cortex and striatum. The expression of fra-1, NGFI-C, egr-3 and Nurr1 did not show any change. The time course of the induction of NGFI-A, NGFI-B, fra-2 and TIS11 was similar to that previously observed with c-fos and the members of the jun family. The present results suggest that NGFI-A, NGFI-B, fra-2 and TIS11 mediate some of the stress-induced changes of the PVN at the level of gene expression. Although the genes of the NGFI/egr family encode structurally similar proteins, they seem to be regulated differentially and thus have diverse roles in regulating gene expression in the brain.
我们之前已经证明，应激会导致大脑中即刻早期基因（IEGs）c-fos和jun基因家族成员的表达迅速且短暂地升高。在此，我们证明了应激对fra-1、fra-2以及最近鉴定出的编码含锌指蛋白的IEGs表达的影响。辣椒素诱导的应激导致下丘脑室旁核中NGFI-A、NGFI-B、fra-2和TIS11迅速且短暂地诱导表达。大脑皮层和纹状体中的NGFI-A mRNA水平也略有增加。fra-1、NGFI-C、egr-3和Nurr1的表达未显示任何变化。NGFI-A、NGFI-B、fra-2和TIS11的诱导时间进程与先前观察到的c-fos和jun家族成员的相似。目前的结果表明，NGFI-A、NGFI-B、fra-2和TIS11在基因表达水平上介导了一些应激诱导的室旁核变化。尽管NGFI/egr家族的基因编码结构相似的蛋白质，但它们似乎受到不同的调节，因此在调节大脑中的基因表达方面具有不同的作用。
