Li K W, van Golen F A, van Minnen J, van Veelen P A, van der Greef J, Geraerts W P
Graduate School Neurosciences Amsterdam, Research Institute Neurosciences Vrije Universiteit, Faculty of Biology, Vrije Universiteit, The Netherlands.
Brain Res Mol Brain Res. 1994 Sep;25(3-4):355-8. doi: 10.1016/0169-328x(94)90172-4.
We used a strategy combining immunodetection, peptide chemistry, and a novel method, direct peptide fingerprinting of neurons and small pieces of nerve by using matrix-assisted laser desorption ionization mass spectrometry, to structurally identify and localize the neuropeptide myomodulin-A in the mollusc, Lymnaea stagnalis. Lymnaea myomodulin appeared to be identical to Aplysia myomodulin-A and is produced by many central neurons, including neurons located in the ventral lobe of the right cerebral ganglion that innervate the penis complex via the penis nerve. Myomodulin-A could also be characterized from the penis complex, and physiological concentrations of the peptide enhanced the relaxation rate of electrically induced contractions of the penis retractor muscle in vitro in a dose-dependent fashion.
我们采用了一种结合免疫检测、肽化学以及一种新方法的策略，即通过基质辅助激光解吸电离质谱对神经元和小块神经进行直接肽指纹图谱分析，来在结构上鉴定和定位软体动物静水椎实螺中的神经肽肌调节素 -A。静水椎实螺的肌调节素似乎与海兔肌调节素 -A相同，并且由许多中枢神经元产生，包括位于右侧脑神经节腹叶通过阴茎神经支配阴茎复合体的神经元。肌调节素 -A也可以从阴茎复合体中鉴定出来，该肽的生理浓度在体外以剂量依赖的方式提高了阴茎牵缩肌电诱导收缩的松弛速率。
