Santama N, Brierley M, Burke J F, Benjamin P R
Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Brighton, United Kingdom.
J Comp Neurol. 1994 Apr 15;342(3):352-65. doi: 10.1002/cne.903420304.
This paper investigates the distribution of four classes of neuropeptides, myomodulin, small cardioactive peptide (SCP), buccalin, and FMRFamide, in central neurons forming the network that underlies feeding behavior in the snail Lymnaea stagnalis. Intracellular dye-marking and immunocytochemical analysis, using antisera to the different classes of peptides, were applied to identified neurons of all three levels of the hierarchy of the circuitry: modulatory interneurons (cerebral giant cells, CGC; slow oscillator, SO), central pattern generator (CPG) interneurons (N1, N2, N3), motoneurons (B1-B10), and their peripheral target organs. Myomodulin immunoreactivity was detected in the CGC interneurons, in the SO, and in ventral N2-type CPG interneurons. Several large buccal motoneurons, the paired B1, B2, B3, B7, and neurons located in the dorsal posterior area (putative B4 cluster types) were also myomodulin immunoreactive. Target organs of buccal motoneurons, the buccal mass, salivary glands, and oesophagus contained myomodulin-immunopositive fibers. SCP appeared in N2-type interneurons and was found colocalized with myomodulin in the B1 and B2 motoneurons. SCP-containing neurons in the B4 cluster area were also detected. The buccal mass and salivary glands exhibited SCP-immunoreactive fibers. Buccalin immunoreactivity was scarce in the buccal ganglia and was identified only in N1-type interneurons and three pairs of dorsal posterior neurons. In the periphery, immunoreactive fibers were localized in the oesophagus only. None of the buccal neuronal types examined revealed immunoreactivity to SEQPDVDDYLRDVVLQSEEPLY ("SEEPLY"), a peptide encoded in the FMRFamide precursor protein of Lymnaea. SEEPLY immunoreactivity was confined to a pair of novel ventral neurons with projections to the laterobuccal nerve innervating the buccal mass. Immunoreactive fibers were also traced in this organ.
本文研究了四种神经肽，即肌动蛋白调节肽、小的心脏活性肽（SCP）、口侧素和FMRF酰胺，在构成椎实螺进食行为基础网络的中枢神经元中的分布。利用针对不同类肽的抗血清，对电路层次结构所有三个水平的已鉴定神经元进行了细胞内染料标记和免疫细胞化学分析：调制性中间神经元（脑巨细胞，CGC；慢振荡器，SO）、中枢模式发生器（CPG）中间神经元（N1、N2、N3）、运动神经元（B1 - B10）及其外周靶器官。在CGC中间神经元、SO以及腹侧N2型CPG中间神经元中检测到肌动蛋白调节肽免疫反应性。几个大型口侧运动神经元，成对的B1、B2、B3、B7以及位于背侧后部区域的神经元（假定的B4簇类型）也具有肌动蛋白调节肽免疫反应性。口侧运动神经元的靶器官，口侧块、唾液腺和食管含有肌动蛋白调节肽免疫阳性纤维。SCP出现在N2型中间神经元中，并在B1和B2运动神经元中与肌动蛋白调节肽共定位。在B4簇区域也检测到含SCP的神经元。口侧块和唾液腺显示出SCP免疫反应性纤维。口侧素免疫反应性在口侧神经节中很少见，仅在N1型中间神经元和三对背侧后部神经元中鉴定到。在周围，免疫反应性纤维仅定位在食管中。所检查的口侧神经元类型均未显示对SEQPDVDDYLRDVVLQSEEPLY（“SEEPLY”）的免疫反应性，SEQPDVDDYLRDVVLQSEEPLY是椎实螺FMRF酰胺前体蛋白中编码的一种肽。SEEPLY免疫反应性局限于一对新的腹侧神经元，其投射到支配口侧块的侧口神经。在该器官中也追踪到了免疫反应性纤维。
