Warbrick E, Glover D
Department of Anatomy and Physiology, University of Dundee, Scotland.
Mol Gen Genet. 1994 Dec 1;245(5):654-7. doi: 10.1007/BF00282229.
We describe a screen to isolate cDNAs encoding Drosophila mitosis inhibitors capable of suppressing the mitotic catastrophe phenotype resulting in Schizosaccharomyces pombe from the combination of the wee1-50 mutation with either a deletion allele of mik1, or with overexpression of cdc25+. One plasmid was isolated which could suppress the temperature sensitive lethality of both these strains. The cDNA in this plasmid encodes a protein highly homologous to the DEAD-box family of ATP-dependent RNA helicases, rather than to protein kinases as might be expected. It is possible that the RNA helicase described here may regulate entry into mitosis by down regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis.
我们描述了一种筛选方法，用于分离编码果蝇有丝分裂抑制剂的cDNA，这些抑制剂能够抑制因wee1 - 50突变与mik1缺失等位基因或cdc25 +过表达相结合而导致粟酒裂殖酵母出现的有丝分裂灾难表型。分离出了一个质粒，它可以抑制这两种菌株的温度敏感致死性。该质粒中的cDNA编码一种与ATP依赖性RNA解旋酶的DEAD-box家族高度同源的蛋白质，而不是如预期的蛋白激酶。这里描述的RNA解旋酶可能通过下调其他基因的表达来调节进入有丝分裂的过程，这些基因的活性可能是进入有丝分裂的限速因素。
