Kholodenko B N, Cascante M, Westerhoff H V
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Mol Cell Biochem. 1994 Apr-May;133-134:313-31. doi: 10.1007/BF01267963.
Various factors appear to control muscle energetics, often in conjunction. This calls for a quantitative approach of the type provided by Metabolic Control Analysis for intermediary metabolism and mitochondrial oxidative phosphorylation. To the extent that direct transfer of high energy phosphates and spatial organization plays a role in muscle energetics however, the standard Metabolic Control Theory does not apply, neither do its theorems regarding control. This chapter develops the Control Theory that does apply to the muscle system. It shows that direct transfer of high energy phosphates bestows a system with enhanced control: the sum of the control exerted by the participating enzymes on the flux of free energy form the mitochondrial matrix to the actinomyosin may well exceed the 100% mandatory for ideal metabolic pathways. It is also shown how sequestration of high energy phosphates may allow for negative control on pathway flux. The new control theory gives methods functionally to diagnose the extent to which channelling and metabolite sequestration occur.
多种因素似乎共同控制着肌肉能量代谢。这就需要采用一种类似于代谢控制分析用于中间代谢和线粒体氧化磷酸化的定量方法。然而，就高能磷酸的直接转移和空间组织在肌肉能量代谢中发挥作用而言，标准的代谢控制理论并不适用，其关于控制的定理也不适用。本章阐述了适用于肌肉系统的控制理论。研究表明，高能磷酸的直接转移赋予系统更强的控制能力：参与其中的酶对从线粒体基质到肌动球蛋白的自由能通量所施加的控制之和很可能超过理想代谢途径所要求的100%。研究还表明，高能磷酸的隔离如何能够对途径通量进行负调控。新的控制理论给出了从功能上诊断通道化和代谢物隔离发生程度的方法。
