Flemmig T F, Miyasaki K T
Poliklinik für Zahnerhaltung und Parodontologie, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
Oral Microbiol Immunol. 1994 Oct;9(5):272-7. doi: 10.1111/j.1399-302x.1994.tb00070.x.
The purpose of this study was to evaluate whether the reduced microbicidal activity of polymorphonuclear leukocytes (neutrophils) in patients with early-onset periodontitis is associated with a deficiency of nonoxidative microbicidal proteins. Neutrophils from 10 patients with early-onset periodontitis and 8 healthy control subjects were assessed for elastase isozymes 1 through 4, cathepsin G isozymes 1 through 4 and defensins (HNP-1, HNP-2 and HNP-3) using cationic and acid urea polyacrylamide gel electrophoresis. The results showed that both the total content and the relative distribution of elastase and cathepsin G isozymes was normal in neutrophils of patients with early-onset periodontitis. However, the HNP-3 content was significantly reduced in neutrophils from patients with generalized early-onset periodontitis. These findings indicate that the impaired microbicidal activities of neutrophils in patients with early-onset periodontitis does not appear to be based on an elastase or cathepsin G abnormality in neutrophils. Due to the high variability of HNP-1 + 2 and HNP-3 in neutrophils of control subjects, the reduced HNP-3 content in neutrophils probably plays a minor role in the pathogenesis of generalized early-onset periodontitis.
本研究的目的是评估早发性牙周炎患者多形核白细胞（中性粒细胞）杀菌活性降低是否与非氧化杀菌蛋白缺乏有关。使用阳离子和酸性尿素聚丙烯酰胺凝胶电泳，对10例早发性牙周炎患者和8名健康对照者的中性粒细胞进行弹性蛋白酶同工酶1至4、组织蛋白酶G同工酶1至4和防御素（HNP-1、HNP-2和HNP-3）评估。结果显示，早发性牙周炎患者中性粒细胞中弹性蛋白酶和组织蛋白酶G同工酶的总含量和相对分布均正常。然而，广泛性早发性牙周炎患者中性粒细胞中的HNP-3含量显著降低。这些发现表明，早发性牙周炎患者中性粒细胞杀菌活性受损似乎并非基于中性粒细胞中弹性蛋白酶或组织蛋白酶G异常。由于对照者中性粒细胞中HNP-1 + 2和HNP-3的变异性较高，中性粒细胞中HNP-3含量降低可能在广泛性早发性牙周炎的发病机制中起次要作用。
