杜氏利什曼原虫和热带利什曼原虫小环DNA的种内和种间多态性
Intra- and interspecific polymorphisms of Leishmania donovani and L. tropica minicircle DNA.
作者信息
Dobner P, Löscher T, Rinder H
机构信息
Abteilung für Infektions- und Tropenmedizin, Universität München, Germany.
出版信息
Parasitol Res. 1994;80(6):474-7. doi: 10.1007/BF00932693.
A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment of Leishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible.
构建了一对简并聚合酶链反应（PCR）引物（LEI-1，TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT；LEI-2，ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC），用于扩增利什曼原虫小环DNA的一个0.15 kb片段。这些引物不仅能扩增种间多态性序列，还能扩增种内多态性序列。单个序列显示种内差异高于种间差异。得出的结论是，单个序列对于物种鉴定的相关性有限。相比之下，当在系统发育图中分析一个包含19个不同序列的数据库时，准确的物种区分是可行的。
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