Cohen L, Mohr R, Chen Y Y, Huang M, Kato R, Dorin D, Tamanoi F, Goga A, Afar D, Rosenberg N
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1662.
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12448-52. doi: 10.1073/pnas.91.26.12448.
We report the characterization of a member of the ras gene family that is overexpressed in cells transformed by abl tyrosine kinase oncogenes. The gene, named kir (for kinase-inducible ras-like), is induced at the transcriptional level. kir mRNA has a rapid turnover and encodes a protein of 33 kDa with guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. kir was cloned by differential screening of genes present in fully malignant versus growth factor-independent cell lines expressing wild-type or mutant forms of BCR/ABL. BCR/ABL and v-Abl induce transcription of the kir gene via specific signaling pathway(s), but kir overexpression alone is not sufficient to mediate transformation.
我们报道了ras基因家族中一个成员的特性，该成员在由abl酪氨酸激酶致癌基因转化的细胞中过表达。这个名为kir（激酶诱导的类ras）的基因在转录水平上被诱导。kir mRNA周转迅速，编码一个33 kDa的具有鸟嘌呤核苷酸结合活性但未检测到内在GTP酶活性的蛋白质。kir是通过对完全恶性与生长因子非依赖性细胞系中存在的基因进行差异筛选而克隆得到的，这些细胞系表达野生型或突变型的BCR/ABL。BCR/ABL和v-Abl通过特定的信号通路诱导kir基因的转录，但单独的kir过表达不足以介导转化。
