Finley R L, Brent R
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12980-4. doi: 10.1073/pnas.91.26.12980.
We characterized interactions between Drosophila melanogaster cell cycle regulatory proteins by a yeast interaction-mating technique. The results were displayed as two-dimensional matrices that revealed individual binary interactions between proteins. Each protein (Cdi, cyclin-dependent kinase interactor) interacted with a distinct spectrum of cyclin-dependent kinases (Cdk) from Drosophila and other organisms. Some Cdis interacted with other Cdis, indicating that these proteins may form trimeric complexes that include Cdks. Similar analysis of interaction matrices may be generally useful in detecting other multiprotein complexes and in establishing connectivity between individual complex members. Moreover, such analysis may also help assign function to newly identified proteins, identify domains involved in protein-protein interactions, and aid the dissection of genetic regulatory networks.
我们通过酵母相互作用交配技术对黑腹果蝇细胞周期调节蛋白之间的相互作用进行了表征。结果以二维矩阵形式呈现，该矩阵揭示了蛋白质之间的个体二元相互作用。每种蛋白质（细胞周期蛋白依赖性激酶相互作用因子Cdi）都与来自果蝇和其他生物体的不同谱的细胞周期蛋白依赖性激酶（Cdk）相互作用。一些Cdi与其他Cdi相互作用，表明这些蛋白质可能形成包含Cdk的三聚体复合物。对相互作用矩阵进行类似分析可能在检测其他多蛋白复合物以及建立各个复合物成员之间的连接性方面普遍有用。此外，这种分析还可能有助于为新鉴定的蛋白质赋予功能、识别参与蛋白质-蛋白质相互作用的结构域，并有助于剖析遗传调控网络。
