Murguía J R, Bellés J M, Serrano R
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Spain.
Science. 1995 Jan 13;267(5195):232-4. doi: 10.1126/science.7809627.
Overexpression of a yeast gene, HAL2, allows the cells to tolerate higher than normal extracellular salt concentrations. HAL2 encodes a 3'(2')5'-bisphosphate nucleotidase that serves to remove the end products of sulfate transfer during cellular metabolism. The enzyme is inhibited by lithium and sodium and is activated by potassium. Metabolic systems that are sensitive to salt, as well as those governing osmolyte synthesis and ion transport, offer routes by which genetic engineering can be used to improve the tolerance of various organisms to salt.
酵母基因HAL2的过表达使细胞能够耐受高于正常水平的细胞外盐浓度。HAL2编码一种3'(2')5'-二磷酸核苷酸酶，其作用是在细胞代谢过程中去除硫酸盐转移的终产物。该酶受到锂和钠的抑制，并被钾激活。对盐敏感的代谢系统，以及那些控制渗透溶质合成和离子转运的系统，为利用基因工程提高各种生物体的耐盐性提供了途径。
