Ziran B H, Pineda S, Pokharna H, Esteki A, Mansour J M, Moskowitz R W
Department of Orthopaedic Surgery, University Hospitals of Cleveland, Case Western Reserve University, Ohio.
Spine (Phila Pa 1976). 1994 Oct 1;19(19):2159-63. doi: 10.1097/00007632-199410000-00005.
The desert sand rat, an animal model for spinal degenerative disc disease, was studied for biomechanical, histologic, and radiographic characteristics. Load deformation curves with peak force and force decay, hematoxylin-eosin-stained sections, and lateral radiographs were correlated.
The hypothesis that the subchondral bone response preceded or contributed to the degenerative disc disease seen in the desert sand rat was tested.
Intervertebral disc disease spontaneously develops in the desert sand rat during its lifetime. Approximately 50% will have significant disc disease by age 18 months. Previous studies have found metabolic and histologic changes associated with intervertebral disc degeneration in the sand rat.
Desert sand rats were killed at 3-, 9-, 15-, and 18-month intervals and tested in Rheometrics Solid Analyzer with nondestructive step strain loading to obtain a load deformation curve. Radiographs and histologic sections of each animal were compared. Peak force and force decay were analyzed by level and by age. Analysis of variance and Tukey's methods were applied to data.
There were no statistically significant differences in biomechanical data. No histologic changes were noted in the study groups up to 18 months of age. Radiographic differences characterized by subchondral bony sclerosis were noted by 18 months of age. No trends were noted when radiographic grades were compared with force data.
The absence of biomechanical histologic changes in the intervertebral disc per se from 3 through 18 months, with radiographic changes in the subchondral bone, supports the hypothesis that the earliest changes in intervertebral disc disease in this animal model are related to the subchondral bone response.
对沙漠沙鼠这一脊柱退行性椎间盘疾病的动物模型进行了生物力学、组织学和影像学特征研究。将具有峰值力和力衰减的载荷变形曲线、苏木精 - 伊红染色切片以及侧位X线片进行关联分析。
检验关于软骨下骨反应先于或导致沙漠沙鼠出现退行性椎间盘疾病的假说。
沙漠沙鼠在其生命周期中会自发发生椎间盘疾病。到18个月龄时，约50%的沙鼠会出现明显的椎间盘疾病。先前的研究已发现沙鼠椎间盘退变相关的代谢和组织学变化。
每隔3个月、9个月、15个月和18个月处死沙漠沙鼠，并在流变学固体分析仪中采用无损阶跃应变加载进行测试，以获得载荷变形曲线。对每只动物的X线片和组织学切片进行比较。按水平和年龄分析峰值力和力衰减。对数据应用方差分析和Tukey方法。
生物力学数据无统计学显著差异。在18个月龄之前，研究组未观察到组织学变化。到18个月龄时，观察到以软骨下骨硬化为特征的影像学差异。将影像学分级与力数据进行比较时，未发现趋势。
在3至18个月期间，椎间盘本身未出现生物力学组织学变化，而软骨下骨出现影像学变化，这支持了在该动物模型中，椎间盘疾病最早的变化与软骨下骨反应相关的假说。
