[The interrelation between the morphological characteristics of the nucleolar apparatus in cultured human embryonic fibroblasts and the proliferative activity of the cells].

A relationship between the number of AgNOR, nucleolar area, the number of nucleoli per nuclei, the number of nucleoli touching nucleolar membrane and the proliferating activity of human embryonal fibroblast culture was investigated. Cell proliferation was arrested by serum deprivation. In 10 days, initiation of proliferation was performed by cessation of serum deprivation. The strongest correlation (r = 0.96) was found between the index of labeling (IL) and the number of nucleoli touching nucleolar membrane. A strong correlation was also observed between AgNOR count, nucleolar area and IL. The number of silver grains in the interphase nucleus decreased slowly and increased quickly, with quick decrease and increase of cell proliferation occurring, respectively. No correlation was found between the number of nucleoli and IL.