Cartman M L, Morris J A, Oates K, Huddart H, Staff W G
Division of Biological Sciences, Lancaster University, United Kingdom.
Ultrastruct Pathol. 1994 Sep-Oct;18(5):461-6. doi: 10.3109/01913129409023220.
Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10(-5) mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and non-neoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.
将人类肿瘤性和非肿瘤性尿路上皮样本立即冷冻，置于 Krebs 盐溶液中孵育后再冷冻，或置于含 10(-5) mol/L 哇巴因的 Krebs 盐溶液中孵育后再冷冻。然后将冷冻标本用冷冻超薄切片机切片，并用低温扫描电子显微镜检查。对尿路上皮层表面细胞进行 X 射线微分析。立即冷冻的肿瘤细胞以及在 Krebs 盐溶液中孵育的肿瘤细胞，其 K+/Na+、K+/P 和 K+/Cl- 比值显著高于非肿瘤细胞，而 Na+/P 和 Cl-/P 比值则低于非肿瘤细胞。在哇巴因中孵育导致肿瘤细胞和非肿瘤细胞的 K+/Na+、K+/P 和 K+/Cl- 比值下降，Na+/P 和 Na+/Cl- 比值上升，并有效消除了它们之间的差异。这些结果与以下概念一致：在肿瘤形成过程中，一个主要事件是 Na+/H+ 交换受到刺激，这会导致对哇巴因敏感的 Na+/K+ ATP 酶泵受到继发性刺激。
