Cartman M L, Morris J A, Huddart H, Staff W G
Division of Biological Sciences, IEBS, Lancaster University, UK.
Br J Urol. 1995 May;75(5):599-603. doi: 10.1111/j.1464-410x.1995.tb07416.x.
To investigate the effect of amiloride preincubation on the intracellular electrolyte levels in neoplastic and non-neoplastic urothelium.
Samples of papillary transitional cell carcinomas (grades 1 and 2) and non-neoplastic human urothelium were either frozen immediately or after pre-incubation with Krebs saline alone or with Krebs saline containing amiloride. The frozen samples were planed in a cryo-ultramicrotome and then examined by low temperature scanning electron microscopy. Intracellular electrolyte levels were measured using X-ray microanalysis.
In the cells frozen immediately the ratios K+/Na+, K+/P and K+/Cl- were significantly increased in neoplastic compared with non-neoplastic urothelial cells (P < 0.001). This difference was also found in the cells pre-incubated with Krebs saline alone but was not present in the cells pre-incubated with amiloride.
These results were consistent with the concept that activation of the amiloride-sensitive Na+/H+ membrane exchanger is a primary event in neoplasia.
研究氨氯吡咪预孵育对肿瘤性和非肿瘤性尿路上皮细胞内电解质水平的影响。
乳头状移行细胞癌（1级和2级）及非肿瘤性人尿路上皮样本，要么立即冷冻，要么先单独用 Krebs 生理盐水预孵育，要么用含氨氯吡咪的 Krebs 生理盐水预孵育。将冷冻样本在低温超薄切片机上切片，然后用低温扫描电子显微镜检查。使用X射线微量分析测量细胞内电解质水平。
与非肿瘤性尿路上皮细胞相比，立即冷冻的肿瘤性尿路上皮细胞中K⁺/Na⁺、K⁺/P和K⁺/Cl⁻比值显著升高（P < 0.001）。单独用Krebs生理盐水预孵育的细胞中也发现了这种差异，但在氨氯吡咪预孵育的细胞中未发现。
这些结果与氨氯吡咪敏感的Na⁺/H⁺膜交换器激活是肿瘤形成中的主要事件这一概念一致。
