Koskelainen A, Hemilä S, Donner K
Laboratory of Physics, Helsinki University of Technology, Espoo, Finland.
Acta Physiol Scand. 1994 Sep;152(1):115-24. doi: 10.1111/j.1748-1716.1994.tb09790.x.
ERG mass photoreceptor responses were recorded across the isolated, aspartate-perfused retina of the frog, Rana temporaria, in order to determine spectral sensitivities of cones. Cone responses were distinguished from rod responses by their faster kinetics, and responses from different cone types were isolated by selective background adaptation. Our main finding is that of a novel short-wavelength sensitive cone population peaking at about 431 nm. Further, we find that the sensitivity spectrum of the dominant long-wavelength sensitive cone population fully accounts for the most common type of photopic ganglion cell spectrum. Both can be described by a nomogram with lambda max = 562 nm. This resolves a long-standing apparent conflict between cone absorbance spectra and ganglion cell sensitivities. Including the 502 nm cones previously described by microspectrophotometry, the frog possesses a collection of cones that could support trichromatic photopic vision.
为了确定视锥细胞的光谱敏感性，在分离的、用天冬氨酸灌注的林蛙视网膜上记录了视网膜电图（ERG）的群体光感受器反应。视锥细胞的反应因其更快的动力学与视杆细胞的反应区分开来，并且通过选择性背景适应分离出不同视锥细胞类型的反应。我们的主要发现是一个新的短波长敏感视锥细胞群体，其峰值约在431纳米。此外，我们发现占主导地位的长波长敏感视锥细胞群体的敏感性光谱完全解释了最常见类型的明视觉神经节细胞光谱。两者都可以用λmax = 562纳米的列线图来描述。这解决了视锥细胞吸收光谱与神经节细胞敏感性之间长期存在的明显冲突。包括先前用显微分光光度法描述的502纳米视锥细胞，林蛙拥有一组可以支持三色明视觉的视锥细胞。
