四氰基对苯二酚介导的用于NADH的流动注射分析电化学传感器与脱氢酶联用。

Tetracyanoquinodimethane-mediated flow injection analysis electrochemical sensor for NADH coupled with dehydrogenase enzymes.

作者信息

Pandey P C

机构信息

Biotechnology Division, National Institute of Standard and Technology, Gaithersburg, Maryland 20899.

出版信息

Anal Biochem. 1994 Sep;221(2):392-6. doi: 10.1006/abio.1994.1431.

DOI:10.1006/abio.1994.1431

PMID:7810883

Abstract

A flow injection analysis (FIA) sensor for the oxidation of NADH is reported. The system utilizes a graphite paste electrode modified with the electroactive material tetracyanoquinodimethane (TCNQ). TCNQ acts as an efficient mediator for the oxidation of NADH to biologically active NAD+. Alcohol dehydrogenase/lactate dehydrogenase and NAD+ were coimmobilized in TCNQ-modified graphite paste using polyethylenimine to develop a FIA sensor for ethanol/lactate. The system responded rapidly with wide linearity. Response curves for ethanol/lactate and NADH are reported.

摘要

报道了一种用于烟酰胺腺嘌呤二核苷酸（NADH）氧化的流动注射分析（FIA）传感器。该系统采用了用电子活性材料四氰基对苯二醌二甲烷（TCNQ）修饰的石墨糊电极。TCNQ作为一种有效的介质，可将NADH氧化为具有生物活性的烟酰胺腺嘌呤二核苷酸（NAD⁺）。使用聚乙烯亚胺将乙醇脱氢酶/乳酸脱氢酶和NAD⁺共固定在TCNQ修饰的石墨糊中，以开发用于乙醇/乳酸的FIA传感器。该系统响应迅速，线性范围宽。报道了乙醇/乳酸和NADH的响应曲线。