Pandey P C, Glazier S, Weetall H H
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.
Anal Biochem. 1993 Oct;214(1):233-7. doi: 10.1006/abio.1993.1482.
A biosensor system using flow injection analysis (FIA) has been developed for the analysis of glucose in human serum. The system consists of the enzyme glucose oxidase incorporated into graphite paste modified with the electroactive material tetracyanoquinodimethane (TCNQ). TCNQ acts as an efficient mediator for oxidation of the reduced enzyme at 200 mV vs Ag/AgCl. The flow injection assay described has detection limits of 2 mM glucose using a 100-microliters sample injection through a 250-microliters sample loop. Data are presented to show the effect of sample injection volume and flow rate on the response of the FIA sensor. The biosensor exhibited excellent reproducibility for 800 injections. The loss of response after 800 injections was due to leaching of TCNQ from the graphite paste. Each assay takes 3 min giving a sample throughput of 20 per hour at a flow rate of 30 ml/h. The sensor was applied to the determination of glucose in human serum. The glucose measurements are in good agreement with those of a commercially available spectrophotometric method. Data showing the effect of interfering substances, ascorbic acid and acetaminophen, on the response of the sensor are also reported.
一种采用流动注射分析（FIA）的生物传感器系统已被开发用于分析人血清中的葡萄糖。该系统由掺入用电子活性材料四氰基对苯二醌二甲烷（TCNQ）修饰的石墨糊中的葡萄糖氧化酶组成。相对于Ag/AgCl，TCNQ在200 mV时作为还原酶氧化的有效媒介。所述流动注射分析通过250微升进样环注入100微升样品时，葡萄糖的检测限为2 mM。给出的数据显示了进样体积和流速对FIA传感器响应的影响。该生物传感器在800次进样中表现出优异的重现性。800次进样后响应的损失是由于TCNQ从石墨糊中浸出。每次分析需要3分钟，在流速为30 ml/h时，每小时样品通量为20个。该传感器应用于人血清中葡萄糖的测定。葡萄糖测量值与市售分光光度法的测量值吻合良好。还报告了显示干扰物质抗坏血酸和对乙酰氨基酚对传感器响应影响的数据。
