Yasuda K, Inagaki N, Yamada Y, Kubota A, Seino S, Seino Y
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Japan.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1556-62. doi: 10.1006/bbrc.1994.2844.
We have isolated a cDNA encoding a gastric inhibitory polypeptide (GIP) receptor from a hamster insulinoma (HIT-T15) cDNA library. The hamster GIP receptor is a 462 amino acid protein having seven transmembrane segments. Expression of recombinant of hamster GIP receptors in Chinese hamster ovary (CHO) cells shows that it binds specifically to GIP with high affinity (IC50 = 9.6 nM) and is positively coupled to adenylate cyclase, but not to phospholipase C. RNA blot analysis reveals that a 3.8-kb GIP receptor mRNA is expressed at high levels in rat pancreatic islets as well as in HIT-T15 cells.
我们从仓鼠胰岛素瘤（HIT-T15）cDNA文库中分离出了一个编码胃抑制多肽（GIP）受体的cDNA。仓鼠GIP受体是一种含有7个跨膜区段的462个氨基酸的蛋白质。在中国仓鼠卵巢（CHO）细胞中重组表达仓鼠GIP受体表明，它以高亲和力（IC50 = 9.6 nM）特异性结合GIP，并且正向偶联至腺苷酸环化酶，但不偶联至磷脂酶C。RNA印迹分析显示，一个3.8 kb的GIP受体mRNA在大鼠胰岛以及HIT-T15细胞中高水平表达。
