Yamada Y, Hayami T, Nakamura K, Kaisaki P J, Someya Y, Wang C Z, Seino S, Seino Y
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Japan.
Genomics. 1995 Oct 10;29(3):773-6. doi: 10.1006/geno.1995.9937.
Gastric inhibitory polypeptide (GIP), which is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta cells and plays a crucial role in the regulation of insulin secretion during the postprandial phase. We have isolated the human gene (GIPR) and cDNA encoding the GIP receptor by a combination of the conventional screening and polymerase chain reaction procedures. Human GIP receptor cDNA encodes a protein of 466 amino acids that is 81.5 and 81.2% identical to the previously cloned hamster and rat GIP receptor, respectively. Hydropathic analysis shows the presence of a signal peptide and seven potential transmembrane domains, a feature characteristic of the VIP/glucagon/secretin receptor family of G protein-coupled receptors. The human GIPR gene is about 13.8 kb long, consists of 14 exons, and carries 17 Alu repeats.
胃抑制性多肽（GIP）由胃肠道释放，可刺激胰腺β细胞分泌胰岛素，在餐后阶段胰岛素分泌的调节中起关键作用。我们通过传统筛选和聚合酶链反应程序相结合的方法，分离出了编码GIP受体的人类基因（GIPR）和cDNA。人类GIP受体cDNA编码一种由466个氨基酸组成的蛋白质，分别与先前克隆的仓鼠和大鼠GIP受体具有81.5%和81.2%的同源性。亲水性分析表明存在一个信号肽和七个潜在的跨膜结构域，这是G蛋白偶联受体的VIP/胰高血糖素/促胰液素受体家族的特征。人类GIPR基因长约13.8 kb，由14个外显子组成，并带有17个Alu重复序列。
