Trenor C, Lin W, Andrews N C
Howard Hughes Medical Institute, Boston, Massachusetts.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1644-50. doi: 10.1006/bbrc.1994.2856.
Menkes disease and Wilson disease are human disorders of copper metabolism. It has recently been shown that both are due to mutations in P-type ATPase copper transport molecules. Related heavy metal transporting ATPases have been described in several strains of bacteria. In an effort to isolate other mammalian metal transporters, we screened a human small intestine library with probes homologous to conserved sequences in the known proteins. Two novel cDNAs were isolated, which encode new members of this family. Surprisingly, they were both of bacterial origin, most likely derived from E. coli sequences transduced during library construction.
门克斯病和威尔逊病是人类铜代谢紊乱疾病。最近研究表明，这两种疾病都是由P型ATP酶铜转运分子的突变引起的。在几种细菌菌株中已发现了相关的重金属转运ATP酶。为了分离出其他哺乳动物金属转运蛋白，我们用与已知蛋白质保守序列同源的探针筛选了一个人类小肠文库。分离出了两个新的cDNA，它们编码该家族的新成员。令人惊讶的是，它们都起源于细菌，很可能来自文库构建过程中被转导的大肠杆菌序列。
