Phung L T, Ajlani G, Haselkorn R
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9651-4. doi: 10.1073/pnas.91.20.9651.
DNA encoding a P-type ATPase was cloned from the cyanobacterium Synechococcus 7942. The cloned ctaA gene encodes a 790-amino acid polypeptide related to the CopA Cu(2+)-uptake ATPase of Enterococcus hirae, to other known P-type ATPases, and to the candidate gene products for the human diseases of copper metabolism, Menkes disease and Wilson disease. Disruption of the single chromosomal gene in Synechococcus 7942 by insertion of an antibiotic-resistance cassette results in a mutant cell line with increased tolerance to Cu2+ compared with the wild type.
从蓝藻聚球藻7942中克隆出编码P型ATP酶的DNA。克隆的ctaA基因编码一种790个氨基酸的多肽，它与平肠球菌的CopA铜摄取ATP酶、其他已知的P型ATP酶以及人类铜代谢疾病（门克斯病和威尔逊病）的候选基因产物相关。通过插入抗生素抗性盒破坏聚球藻7942中的单个染色体基因，产生了一个与野生型相比对Cu2+耐受性增强的突变细胞系。
