Ohkura N, Hijikuro M, Yamamoto A, Miki K
Terumo R&D Center, Kanagawa, Japan.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1959-65. doi: 10.1006/bbrc.1994.2900.
Brain neuronal cells in primary culture provide one system for the analysis of neuronal development, including apoptosis. By PCR amplification, with primers designed from conserved sequences among nuclear receptors, a novel member of the thyroid/steroid receptor superfamily was identified from forebrain neuronal cells undergoing apoptosis and designated as NOR-1 (neuron derived orphan receptor). A cDNA clone of NOR-1 encodes a 68kDa, 628 amino acid residue-containing protein, with an amino acid sequence highly homologous to the Nur77 family in its DNA-binding domain and moderately so in its putative ligand-binding domain. Using mobility shift assay, we showed that the NOR-1 protein binds to the B1a response-element which has been identified as the target sequence of the Nur77 family, suggesting that three members of the Nur77 family may transactivate common target gene(s) at different situations.
原代培养的脑神经元细胞为分析神经元发育（包括细胞凋亡）提供了一个系统。通过PCR扩增，利用从核受体保守序列设计的引物，从正在经历凋亡的前脑神经元细胞中鉴定出甲状腺/类固醇受体超家族的一个新成员，并将其命名为NOR-1（神经元衍生孤儿受体）。NOR-1的cDNA克隆编码一个含628个氨基酸残基的68kDa蛋白，其氨基酸序列在DNA结合结构域与Nur77家族高度同源，在假定的配体结合结构域中度同源。使用迁移率变动分析，我们表明NOR-1蛋白与已被确定为Nur77家族靶序列的B1a反应元件结合，这表明Nur77家族的三个成员可能在不同情况下反式激活共同的靶基因。
