Hirose T, Smith R J, Jetten A M
Cell Biology Section, Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Biochem Biophys Res Commun. 1994 Dec 30;205(3):1976-83. doi: 10.1006/bbrc.1994.2902.
In this study, we describe the identification and cloning of a novel member of the nuclear receptor superfamily. This orphan receptor, referred to as ROR gamma, belongs to the ROR/RZR subfamily. The open reading frame of ROR gamma encodes a protein of 560 amino acid residues with a predicted molecular mass of 63 kD. The amino acid sequence of ROR gamma exhibits a 50 and 51% identity with those of ROR alpha/RZR alpha and RZR beta, respectively, whereas the DNA-binding domains were 89% identical. ROR gamma was localized on human chromosome 1. Northern blot analysis using RNA from multiple tissues indicated that ROR gamma is expressed in several tissues but is most highly expressed in skeletal muscle.
在本研究中，我们描述了核受体超家族一个新成员的鉴定与克隆。这个孤儿受体被称为RORγ，属于ROR/RZR亚家族。RORγ的开放阅读框编码一个由560个氨基酸残基组成的蛋白质，预测分子量为63kD。RORγ的氨基酸序列与RORα/RZRα和RZRβ的氨基酸序列分别具有50%和51%的同一性，而其DNA结合结构域的同一性为89%。RORγ定位于人类1号染色体。使用来自多个组织的RNA进行的Northern印迹分析表明，RORγ在多个组织中表达，但在骨骼肌中表达最高。
