Schouler C, Ehrlich S D, Chopin M C
Laboratoire de Génétique, Microbienne, INRA, Jouy-en-Josas, France.
Microbiology (Reading). 1994 Nov;140 ( Pt 11):3061-9. doi: 10.1099/13500872-140-11-3061.
bIL67 is a broad-host-range prolate-headed phage that is active against Lactococcus cells. The complete phage genome sequence of 22195 bp was established. Thirty-seven open reading frames (ORFs) organized in two clusters were identified. Functions were assigned to the putative products of six of the ORFs on the basis of comparison of the deduced amino acid sequences to known proteins, analysis of structural features of the proteins and search for conserved motifs. These were a DNA polymerase, a protein involved in recombination, a lysin, a terminase subunit, a structural protein and a holin.
bIL67是一种具有广泛宿主范围的长头噬菌体,对乳球菌细胞具有活性。已确定了22195 bp的完整噬菌体基因组序列。鉴定出37个开放阅读框(ORF),它们分为两个簇。根据推导的氨基酸序列与已知蛋白质的比较、蛋白质结构特征分析以及保守基序搜索,为其中6个ORF的推定产物赋予了功能。这些功能分别是DNA聚合酶、参与重组的蛋白质、溶菌酶、末端酶亚基、结构蛋白和穿孔素。