Ayabe H, Tomita M, Kawahara K, Tagawa Y, Tsuji H, Akamine S
First Department of Surgery, Nagasaki University School of Medicine, Japan.
Lung Cancer. 1994 Sep;11(3-4):201-8. doi: 10.1016/0169-5002(94)90540-1.
Nuclear DNA contents were measured using a flow cytometry technique in non-small cell lung carcinomas and differences in ploidy patterns were compared between primary lung carcinomas and metastatic lymph nodes. Negative node lung cancer revealed diploidy in 82.6% of the 224 non-small cell lung cancers, in contrast with 19.5% in positive node lung cancer. In multi-stemline cells, a high incidence of nodal involvement was seen when compared with single stemline cells. The more the DNA indices increased, the more the lymph nodes were seen to be extensively involved. Furthermore, intratumoral heterogeneity was evaluated in terms of n-categories. In conclusion, it is suggested that nodal metastasis may be caused by tumor cells with high DNA indices in lung carcinomas, in particular for multi-stemline cells.
采用流式细胞术测量非小细胞肺癌的核DNA含量，并比较原发性肺癌与转移性淋巴结的倍体模式差异。在224例非小细胞肺癌中，阴性淋巴结肺癌的二倍体率为82.6%，而阳性淋巴结肺癌为19.5%。在多干系细胞中，与单干系细胞相比，淋巴结受累的发生率较高。DNA指数增加越多，可见淋巴结广泛受累的情况越多。此外，根据n分类评估肿瘤内异质性。总之，提示肺癌中具有高DNA指数的肿瘤细胞可能导致淋巴结转移，尤其是多干系细胞。
