Sara A, el-Naggar A K
Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Am J Clin Pathol. 1991 Sep;96(3):311-7. doi: 10.1093/ajcp/96.3.311.
Twenty-eight resected primary non-small cell lung carcinomas were studied for intratumoral DNA variability by flow cytometry (FCM). Three separate tissue specimens from each resected tumor were divided equally for FCM analysis and histologic evaluation. FCM analysis also was performed on fine-needle aspirates (FNAs) from the center of the resected tumor. Histologically, there were 8 squamous carcinomas, 19 adenocarcinomas, and 1 large cell carcinoma. Twenty-three tumors (82%) were aneuploid, and 5 (18%) were diploid. The DNA index in aneuploid neoplasms ranged from 0.91 to 3.30 (mean, 1.64). All 5 diploid and 19 (83%) of the (23) aneuploid neoplasms manifested intratumoral DNA stability. Four (17%) of the aneuploid tumors showed regional DNA heterogeneity expressed as additional stemlines in at least one sample. The FNA yield was sufficient in 21 cases and inadequate for complete analysis in 7 cases. In general, good correlation between FNA and tissue analysis was obtained. However, in three of the aneuploid neoplasms, FNA materials did not reveal an additional nondominant stemline, as noted in the tissue specimens. The authors attribute this finding to a dilutional factor in the aspiration material. The authors conclude that most non-small cell lung carcinomas express DNA stability; FNA provides adequate cellular material for FCM in most cases.
对28例手术切除的原发性非小细胞肺癌进行了流式细胞术（FCM）检测，以研究肿瘤内DNA的变异性。将每个切除肿瘤的三个独立组织标本平均分为两份，分别用于FCM分析和组织学评估。还对切除肿瘤中心的细针穿刺抽吸物（FNA）进行了FCM分析。组织学上，有8例鳞状细胞癌、19例腺癌和1例大细胞癌。23例肿瘤（82%）为非整倍体，5例（18%）为二倍体。非整倍体肿瘤的DNA指数范围为0.91至3.30（平均为1.64）。所有5例二倍体肿瘤和23例非整倍体肿瘤中的19例（83%）表现出肿瘤内DNA稳定性。4例（17%）非整倍体肿瘤显示出区域DNA异质性，表现为至少一个样本中存在额外的干系。21例FNA取材充足，7例取材不足，无法进行完整分析。总体而言，FNA与组织分析之间具有良好的相关性。然而，在3例非整倍体肿瘤中，FNA材料未显示出组织标本中所观察到的额外非主导干系。作者将这一发现归因于抽吸材料中的稀释因素。作者得出结论，大多数非小细胞肺癌表现出DNA稳定性；在大多数情况下，FNA可为FCM提供足够的细胞材料。
