Lin J Y, Pan J T
Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Brain Res Bull. 1994;35(3):241-6. doi: 10.1016/0361-9230(94)90129-5.
The effects of bombesin, gastrin-releasing peptide, neuromedin C, ranatensin, and neuromedin B on hypothalamic arcuate neurons were tested in this study using extracellular single-unit recording in fresh brain tissue slices. Adult ovariectomized Sprague-Dawley rats were used for preparation of brain slices. All bombesin-like peptides in pmol ranges exhibited potent stimulatory effects on the firing of arcuate neurons, i.e., gastrin-releasing peptide stimulated 90.9% (n = 22), bombesin 78.0% (n = 41), neuromedin C 63.2% (n = 19), ranatensin 58.0% (n = 22), and neuromedin B 50.0% (n = 6) of arcuate neurons tested. Pretreatments with either [Leu13-psi(CH2NH)-Leu14]-bombesin or [D-Phe6,Des-Met14]-bombesin6-14 ethylamide, two bombesin antagonists, significantly blocked most of the actions of bombesin-like peptides tested. The present results further support the notion that bombesin-like peptides may play a significant role in the arcuate nucleus.
在本研究中，利用新鲜脑组织切片的细胞外单单位记录技术，测试了蛙皮素、胃泌素释放肽、神经介素C、蛙皮降压肽和神经介素B对下丘脑弓状核神经元的影响。成年去卵巢的斯普拉格-道利大鼠用于制备脑切片。所有皮摩尔范围内的蛙皮素样肽对弓状核神经元的放电均表现出强烈的刺激作用，即胃泌素释放肽刺激了90.9%（n = 22）的受试弓状核神经元，蛙皮素刺激了78.0%（n = 41），神经介素C刺激了63.2%（n = 19），蛙皮降压肽刺激了58.0%（n = 22），神经介素B刺激了50.0%（n = 6）。用两种蛙皮素拮抗剂[Leu13-psi(CH2NH)-Leu14]-蛙皮素或[D-Phe6,Des-Met14]-蛙皮素6-14乙酰胺预处理，可显著阻断所测试的大多数蛙皮素样肽的作用。目前的结果进一步支持了蛙皮素样肽可能在弓状核中发挥重要作用这一观点。
