Lin J Y, Pan J T
Institute of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Brain Res Bull. 1993;30(1-2):177-80. doi: 10.1016/0361-9230(93)90056-h.
Using extracellular single-unit recording in brain tissue slices, the effects of bombesin (Bomb), neurotensin (NT), and their analogs on hypothalamic arcuate (ARC) neurons were tested in this study. Adult ovariectomized Sprague-Dawley rats were used for obtaining the brain slices. Both Bomb and NT in pmol ranges stimulated nearly 70% of ARC neurons tested and the effects were all very significant. [D-Trp11]-NT, a NT analog, behaved more like an analog of NT instead of an antagonist, although [Leu13-psi(CH2NH)-Leu14]-Bomb, a Bomb receptor antagonist, could not block most of Bomb's effects on ARC neurons. The significant effects of Bomb and NT shown in this report indicate that both peptides may play significant roles in the neuroendocrine control system.
在本研究中，利用脑组织切片的细胞外单单位记录技术，检测了蛙皮素（Bomb）、神经降压素（NT）及其类似物对下丘脑弓状核（ARC）神经元的影响。采用成年去卵巢的Sprague-Dawley大鼠获取脑切片。皮摩尔浓度范围的Bomb和NT均可刺激近70%的受试ARC神经元，且作用均非常显著。NT类似物[D-Trp11]-NT表现得更像是NT的类似物而非拮抗剂，尽管Bomb受体拮抗剂[Leu13-psi(CH2NH)-Leu14]-Bomb无法阻断Bomb对ARC神经元的大部分作用。本报告中显示的Bomb和NT的显著作用表明，这两种肽可能在神经内分泌控制系统中发挥重要作用。
