Luque de Castro M D, Quiles R, Fernández-Romero J M, Fernández E
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Clin Chem. 1995 Jan;41(1):99-102.
An automated method for the determination of inorganic phosphate based on flow-injection analysis and on the use of immobilized enzymes is reported. The method features a linear range between 0.1 and 20 mumol/L with a CV < 2.1% and 3.4% for the within-run and between-run studies, respectively, and a sampling throughput of 40 h-1. The sensitivity of the method makes a 1:250 dilution of the serum samples feasible, thus making undetectable the interferences from analytes commonly present in serum. The method shows an excellent correlation with conventional automated analyzers based on the same enzymatic reaction (Hitachi, r = 0.988) but with the catalyst in solution, and with the Kodak Ektachem method (r = 0.974) based on the use of dry reagents and formation of the phosphomolybdo heteropolyacid.
报道了一种基于流动注射分析和固定化酶的无机磷酸盐自动化测定方法。该方法的线性范围为0.1至20 μmol/L，批内和批间研究的变异系数（CV）分别<2.1%和3.4%，采样通量为40次/小时。该方法的灵敏度使血清样本1:250的稀释成为可能，从而使血清中常见分析物的干扰不可检测。该方法与基于相同酶促反应（日立，r = 0.988）但催化剂在溶液中的传统自动化分析仪，以及基于使用干试剂和磷钼杂多酸形成的柯达Ektachem方法（r = 0.974）具有极好的相关性。
