聚合酶链反应（PCR）与培养法在检测泌尿生殖系统感染患者临床样本中解脲脲原体的比较。
Comparison of PCR with culture for detection of Ureaplasma urealyticum in clinical samples from patients with urogenital infections.
作者信息
Teng K, Li M, Yu W, Li H, Shen D, Liu D
机构信息
Department of Medical Genetics, Western Region Hospital, Urumqi, Xinjian, People's Republic of China.
出版信息
J Clin Microbiol. 1994 Sep;32(9):2232-4. doi: 10.1128/jcm.32.9.2232-2234.1994.
PCR was compared with culture for the detection of Ureaplasma urealyticum in 50 specimens, including sperm, urine, and prostate secretions, from hospital patients with urogenital infections. Five positive and a further four doubtful diagnoses were made by culture, whereas PCR detected U. urealyticum in 12 samples. PCR also was faster than culturing. The increased sensitivity and shorter time requirement of PCR support its further development for the diagnosis of U. urealyticum infection.
对50份来自患有泌尿生殖系统感染的医院患者的标本（包括精液、尿液和前列腺分泌物）进行检测，比较了聚合酶链反应（PCR）与培养法检测解脲脲原体的效果。培养法做出了5例阳性诊断和另外4例可疑诊断，而PCR在12份样本中检测到了解脲脲原体。PCR检测速度也比培养法更快。PCR更高的灵敏度和更短的检测时间需求支持其在解脲脲原体感染诊断方面的进一步发展。
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