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采用RNA探针杂交酶免疫分析法检测人细小病毒B19 DNA聚合酶链反应产物

Detection of human parvovirus B19 DNA PCR products by RNA probe hybridization enzyme immunoassay.

作者信息

Erdman D D, Durigon E L, Holloway B P

机构信息

Respiratory and Enterovirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia.

出版信息

J Clin Microbiol. 1994 Sep;32(9):2295-8. doi: 10.1128/jcm.32.9.2295-2298.1994.

DOI:10.1128/jcm.32.9.2295-2298.1994
PMID:7814562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263987/
Abstract

We have developed an RNA probe hybridization enzyme immunoassay for detection of human parvovirus B19 PCR-amplified DNA. The assay is easy to perform and increases assay sensitivity without the added inconvenience and risk of false-positive results associated with nested PCR.

摘要

我们开发了一种RNA探针杂交酶免疫分析法，用于检测人细小病毒B19的PCR扩增DNA。该分析方法易于操作，提高了检测灵敏度，同时避免了与巢式PCR相关的额外不便和假阳性结果风险。

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J Clin Microbiol. 2002 Jan;40(1):111-6. doi: 10.1128/JCM.40.1.111-116.2002.
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J Clin Microbiol. 2001 Dec;39(12):4413-9. doi: 10.1128/JCM.39.12.4413-4419.2001.

本文引用的文献

1
Characterization of a nested polymerase chain reaction assay for detection of parvovirus B19.用于检测细小病毒B19的巢式聚合酶链反应分析方法的特性鉴定
J Clin Microbiol. 1993 Mar;31(3):540-6. doi: 10.1128/jcm.31.3.540-546.1993.
2
Nested polymerase chain reaction assay for the detection of B19 parvovirus DNA in human immunodeficiency virus patients.用于检测人类免疫缺陷病毒患者中B19细小病毒DNA的巢式聚合酶链反应测定法。
J Med Virol. 1993 Jun;40(2):157-60. doi: 10.1002/jmv.1890400214.
3
Polymerase chain reaction for synthesis of digoxigenin-labelled DNA probe: application to parvovirus B19 and to polyomavirus BK.
用于合成地高辛标记DNA探针的聚合酶链反应：应用于细小病毒B19和BK多瘤病毒
Mol Cell Probes. 1993 Feb;7(1):55-60. doi: 10.1006/mcpr.1993.1007.
4
Human parvovirus B19 infections: routine diagnosis by a new nested polymerase chain reaction assay.人细小病毒B19感染：采用新型巢式聚合酶链反应检测法进行常规诊断
J Med Virol. 1993 Jul;40(3):228-34. doi: 10.1002/jmv.1890400311.
5
Multiple primer pairs for polymerase chain reaction (PCR) amplification of human parvovirus B19 DNA.用于聚合酶链反应（PCR）扩增人细小病毒B19 DNA的多对引物。
J Virol Methods. 1993 Oct;44(2-3):155-65. doi: 10.1016/0166-0934(93)90051-r.
6
Possible false-positive results for parvovirus B19 IgM antibodies.细小病毒B19 IgM抗体可能出现假阳性结果。
S Afr Med J. 1993 Jun;83(6):441.
7
Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV).用于人类血清细小病毒样病毒（SPLV）的单克隆抗体诊断检测法。
J Hyg (Lond). 1983 Aug;91(1):113-30. doi: 10.1017/s0022172400060095.
8
Detection of human parvovirus using a molecularly cloned probe.
J Med Virol. 1985 Feb;15(2):173-81. doi: 10.1002/jmv.1890150210.
9
Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis.从再生障碍性贫血危象患儿血清中分离出的人细小病毒B19的核苷酸序列和基因组结构
J Virol. 1986 Jun;58(3):921-36. doi: 10.1128/JVI.58.3.921-936.1986.
10
Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测人细小病毒B19的抗体和抗原。
J Clin Microbiol. 1986 Oct;24(4):522-6. doi: 10.1128/jcm.24.4.522-526.1986.