Takahashi K, Takenaka M, Ishizuka B
Hiroshima Assisted Reproductive Technology Clinic.
Nihon Sanka Fujinka Gakkai Zasshi. 1994 Oct;46(10):1009-12.
Assisted hatching (AHA) techniques were used in 67 patients who had repeatedly failed to achieve conception by conventional IVF-ET in spite of replacement by good quality embryos. The age of the female patients ranged from 28 to 44 years and the causes of their infertility included tubal factors (n = 33), male infertility (16), endometriosis (8) and unexplained causes (21). Zona drilling with acid Tyrode's solution was performed on 248 embryos in 88 cycles approximately 72 hours after oocyte collection. One to 4 embryos were transferred to the uterus 1 to 4 hours later. Twenty-four clinical pregnancies were established (27.3%) and the implantation rate was 15.3% (38/248). These results were both significantly higher than those for our conventional IVF-ET (22.0% pregnancy rate and 10.7% implantation rate) (p < 0.05). Eleven babies have been born normally and there are 16 current pregnancies. These preliminary findings suggest that AHA might be beneficial for patients in whom conventional IVF-ET had repeatedly failed.
对于67例尽管移植了优质胚胎但常规体外受精-胚胎移植（IVF-ET）反复未能受孕的患者，采用了辅助孵化（AHA）技术。女性患者年龄在28至44岁之间，其不孕原因包括输卵管因素（n = 33）、男性不育（16例）、子宫内膜异位症（8例）和不明原因（21例）。在卵母细胞采集后约72小时，对88个周期中的248个胚胎进行了用酸性台氏液打孔。1至4小时后将1至4个胚胎移植到子宫。共确立了24例临床妊娠（27.3%），着床率为15.3%（38/248）。这些结果均显著高于我们常规IVF-ET的结果（妊娠率22.0%，着床率10.7%）（p < 0.05）。已有11名婴儿正常出生，目前有16例妊娠。这些初步研究结果表明，AHA可能对常规IVF-ET反复失败的患者有益。
