Lehtonen L, Korvenranta H, Eerola E
Department of Pediatrics, Turku University Hospital, Finland.
J Pediatr Gastroenterol Nutr. 1994 Oct;19(3):310-4. doi: 10.1097/00005176-199410000-00009.
To find out whether intestinal microflora in colicky infants is different from that in noncolicky controls, stool samples were collected from colicky infants during colic (n = 55) and at the age of 3 months (n = 46) and compared with samples from age-matched controls (n = 49 and n = 45, respectively). The samples were cultured on several selective and unselective aerobic and anaerobic culture agars, and gas-liquid chromatography of bacterial cellular fatty acids was used to produce fatty-acid profiles of the stool samples. In quantitative bacterial cultures, no differences were found between the colicky and control groups in the amounts of each bacterium. The colicky infants were more frequently colonized with Clostridium difficile during the time of colic than were the age-matched controls. This difference disappeared by age 3 months. The fatty-acid profiles did not differ between the colicky and control groups as a whole at the time of colicky symptoms. At age 3 months, a difference in fatty-acid profiles was found between the colicky infants who had suffered from severe colic and the control infants. The fatty-acid profiles were also influenced by the age of the infant, the mode of delivery, antimicrobial drugs taken by the mother during delivery, and breast-feeding and type of feeding. In conclusion, no difference in intestinal microflora was found between the colicky infants at the time of colic and the controls. However, a difference in bacterial cellular fatty-acid profiles at the age of 3 months was found that correlated with severe infantile colic. This difference may contribute to the cause(s) of colic, or it may be secondary to the colic, which may influence the microbial environment of the intestine.
为了探究患腹绞痛婴儿的肠道微生物群是否与无腹绞痛的对照组婴儿不同，研究人员收集了腹绞痛婴儿在腹绞痛发作期间（n = 55）和3个月大时（n = 46）的粪便样本，并与年龄匹配的对照组婴儿（分别为n = 49和n = 45）的样本进行比较。这些样本在几种选择性和非选择性需氧及厌氧培养基上进行培养，同时采用细菌细胞脂肪酸气液色谱法来生成粪便样本的脂肪酸谱。在定量细菌培养中，腹绞痛组和对照组在每种细菌的数量上未发现差异。与年龄匹配的对照组相比，腹绞痛婴儿在腹绞痛发作期间感染艰难梭菌的频率更高。到3个月大时，这种差异消失。在腹绞痛症状出现时，腹绞痛组和对照组的整体脂肪酸谱并无差异。在3个月大时，发现患有严重腹绞痛的腹绞痛婴儿与对照婴儿的脂肪酸谱存在差异。脂肪酸谱还受到婴儿年龄、分娩方式、母亲在分娩期间服用的抗菌药物以及母乳喂养和喂养类型的影响。总之，在腹绞痛发作时，腹绞痛婴儿与对照组婴儿的肠道微生物群未发现差异。然而，在3个月大时发现细菌细胞脂肪酸谱存在差异，这与严重婴儿腹绞痛相关。这种差异可能是腹绞痛的病因之一，也可能是腹绞痛的继发结果，而腹绞痛可能会影响肠道的微生物环境。
