核糖体肽基转移酶+的荧光2'（3'）-O-氨酰基核苷-受体底物
Fluorescent 2'(3')-O-aminoacylnucleosides-acceptor substrates for ribosomal peptidyltransferase+.
作者信息
Chládek S, Ringer D, Abraham E M
出版信息
Nucleic Acids Res. 1976 May;3(5):1215-24. doi: 10.1093/nar/3.5.1215.
Abstract
2'(3')-O-L-Phenylalanylderivatives of fluorescent 1,N6-ethenoadenosine and 3,N4-ethenocytidine were prepared by chemical synthesis. Both compounds are good acceptor substrates in ribosomal peptidyltransferase reactions. Since these compounds cannot form Watson-Crick base pairs, the results indicate that the terminal aminoacyladenosine unit of AA-tRNA is bound to ribosomal protein on the acceptor site of peptidyltransferase and not to rRNA.
摘要
通过化学合成制备了荧光1,N6-乙烯腺苷和3,N4-乙烯胞苷的2'(3')-O-L-苯丙氨酰衍生物。这两种化合物在核糖体肽基转移酶反应中都是良好的受体底物。由于这些化合物不能形成沃森-克里克碱基对，结果表明，氨酰-tRNA的末端氨酰腺苷单元在肽基转移酶的受体位点与核糖体蛋白结合，而不是与rRNA结合。
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Substrate specificity of ribosomal peptidyl transferase: 2'(3')-O-aminoacyl nucleosides as acceptors of the peptide chain on the amino acid site.核糖体肽基转移酶的底物特异性：2'（3'）-O-氨酰基核苷作为氨基酸位点上肽链的受体。
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