来自大肠杆菌突变体的核糖体及核糖体前体的肽基转移酶活性
Peptidyltransferase activity of ribosomes and a ribosome precursor from a mutant of Escherichia coli.
作者信息
Sims P F, Wild D G
出版信息
Biochem J. 1976 Dec 15;160(3):721-6. doi: 10.1042/bj1600721.
Abstract
Escherichia coli strain 15-28 is a mutant with a defect in ribosome synthesis that caused the accumulation of ribonucleoprotein ('47S') particles during exponential growth. These particles are precursors to 50S ribosomes that lack three ribosomal proteins. Peptidyltransferase activity and binding at the peptidyl site of the peptidyltransferase centre are greatly decreased in 47S particles. Both these activities are lower in the 50S and 70S ribosomes of strain 15-28 than in its parent. Unusual assembly of the larger ribosomal subunit in strain 15-28 may produce completed ribosomes with diminished biological activity.
摘要
大肠杆菌菌株15 - 28是一种核糖体合成存在缺陷的突变体，在指数生长期会导致核糖核蛋白（“47S”）颗粒的积累。这些颗粒是缺乏三种核糖体蛋白的50S核糖体的前体。47S颗粒中的肽基转移酶活性以及在肽基转移酶中心肽基位点的结合能力大幅降低。菌株15 - 28的50S和70S核糖体中的这两种活性均低于其亲本。菌株15 - 28中较大核糖体亚基的异常组装可能会产生生物活性降低的完整核糖体。
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