Hac1：一种与CRE基序结合的新型酵母碱性亮氨酸拉链蛋白，是粟酒裂殖酵母cdc10突变体的多拷贝抑制因子。

Hac1: a novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdc10 mutant of Schizosaccharomyces pombe.

作者信息

Nojima H, Leem S H, Araki H, Sakai A, Nakashima N, Kanaoka Y, Ono Y

机构信息

Department of Molecular Genetics, Osaka University, Japan.

出版信息

Nucleic Acids Res. 1994 Dec 11;22(24):5279-88. doi: 10.1093/nar/22.24.5279.

We cloned by phenotypic complementation a novel Saccharomyces cerevisiae's multicopy suppressor of the Schizosaccharomyces pombe cdc10-129 mutant which we call HAC1, an acronym of 'homologous to ATF/CREB 1'. It encodes a bZIP (basic-leucine zipper) protein of 230 amino acids with close homology to the mammalian ATF/CREB transcription factor and gel-retardation assays showed that it binds specifically to the CRE motif. HAC1 is not essential for viability. However, the hac1 disruptant becomes caffeine sensitive, which is suppressed by multicopy expression of the yeast PDE2 (Phosphodiesterase 2) gene. Although the mRNA level of HAC1 is almost constitutive throughout the cell cycle, it fluctuates during meiosis. The upstream region of the HAC1 gene contains a T4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. These results suggest that HAC1 may also be one of the meiotic genes.

我们通过表型互补克隆了一种新型的酿酒酵母多拷贝抑制因子，它可抑制粟酒裂殖酵母cdc10 - 129突变体，我们将其命名为HAC1，是“与ATF/CREB 1同源”的首字母缩写。它编码一种由230个氨基酸组成的bZIP（碱性亮氨酸拉链）蛋白，与哺乳动物的ATF/CREB转录因子具有高度同源性，凝胶阻滞试验表明它能特异性结合CRE基序。HAC1对细胞生存力并非必需。然而，hac1缺失突变体对咖啡因敏感，酵母PDE2（磷酸二酯酶2）基因的多拷贝表达可抑制这种敏感性。尽管HAC1的mRNA水平在整个细胞周期中几乎是组成型的，但在减数分裂过程中会发生波动。HAC1基因的上游区域包含一个T4C位点、一个URS（上游阻遏序列）和一个TR（富含T）盒状序列，这些序列存在于许多减数分裂基因的上游。这些结果表明，HAC1可能也是减数分裂基因之一。