[在间接免疫荧光试验中使用大鼠肾小球基底膜（GBM）作为抗原检测人抗GBM抗体（作者译）]

[Use of rat glomerular basement membrane (GBM) as antigen in indirect imunofluorescence test for detection of human anti-GBM antibodies (author's transl)].

作者信息

De Almeida D B, De Carvalho Anselmo M A, Habermann F

出版信息

Rev Bras Pesqui Med Biol. 1976 Mar-Jun;9(2-3):145-9.

Abstract

UNLABELLED

The purpose of this paper is to verify if we may use the rat glomerular basement membrane (GBM) as antigen, in indirect immunofluorescent test in isolated GBM (IIT-BGM) to detect human anti GBM antibodies. Rabbit anti-human GBM serum (A-H-GBMS) were obtained by rabbit's immunization with human GBM or rat GBM respectively. The content of anti-GBM antibodies in rabbit's sera was determined in IIT-GBM. The titer of the A-H-GBMS was 1/10 when determined in IIT-GBM performed in human GBM and only weakly positive in IIT-GBM performed in rat GBM. The titer of A-R-GBMS was 1/60 and 1/20 when determined respectively in rat GBM and in human GBM. All the reactions performed with sera absorbed with autologous GBM were negative; the absorption of sera with heterologous GBM reduced sera titer.

IN CONCLUSION

a) rat and human GBM have common and different antigenic components; b) to detect human anti-GBM antibodies we must not employ rat GBM in IIT-GBM.

摘要

未标注

本文的目的是验证在分离的肾小球基底膜间接免疫荧光试验（IIT - BGM）中，是否可以使用大鼠肾小球基底膜（GBM）作为抗原检测人抗GBM抗体。分别用人GBM或大鼠GBM免疫兔，获得兔抗人GBM血清（A - H - GBMS）。在IIT - GBM中测定兔血清中的抗GBM抗体含量。在用人GBM进行的IIT - GBM中测定时，A - H - GBMS的效价为1/10，而在大鼠GBM进行的IIT - GBM中仅呈弱阳性。在大鼠GBM和人GBM中分别测定时，A - R - GBMS的效价为1/60和1/20。用自体GBM吸收的血清进行的所有反应均为阴性；用异源GBM吸收血清会降低血清效价。