Boĭko O V, Manukhin B N
Zh Evol Biokhim Fiziol. 1994 Jan-Feb;30(1):82-7.
Studies have been made of the effect of serotonin and tryptamine on contractile activity of nerve-free smooth muscle strip from the amnion of 7-19-day chick embryos. It was shown that stimulatory effect of indolamines depends on the age of embryos. Within 7-9 days of incubation, there was a delay in the onset of the response, the sensitivity being rather low (threshold dose for serotonin-5 microM); prolonged washing before each application of the drugs was usually necessary. At the 13-15th day, the reaction-becomes rather quick, being easily abolished by washing procedure; the sensitivity and the level of maximum reaction increase (for serotonin, mean Kd and Pmax values were equal to 0.3 microM and 62.2 mg/mg tissue respectively). Developmental changes in responses to tryptamine are similar to those described for serotonin. The effectiveness of serotonin at the 7-9th day of incubation is significantly lower than that of Ach, whereas at the 13-15th day the value of Kd is similar to that for Ach, Pmax being often higher than for the latter. The data obtained are discussed in relation to proliferation changes in amniotic tissue.
已有研究探讨了血清素和色胺对7至19日龄鸡胚羊膜无神经平滑肌条收缩活性的影响。结果表明，吲哚胺类物质的刺激作用取决于胚胎的年龄。在孵化7至9天时，反应起始延迟，敏感性较低（血清素的阈值剂量为5微摩尔）；每次用药前通常需要长时间冲洗。在第13至15天时，反应变得相当迅速，很容易被冲洗程序消除；敏感性和最大反应水平增加（血清素的平均解离常数Kd和最大反应值Pmax分别等于0.3微摩尔和62.2毫克/毫克组织）。对色胺反应的发育变化与血清素相似。孵化7至9天时血清素的效力明显低于乙酰胆碱，而在第13至15天时，解离常数Kd的值与乙酰胆碱相似，最大反应值Pmax通常高于后者。结合羊膜组织的增殖变化对所获得的数据进行了讨论。
