Nechaeva M V, Zhurkovich I K, Kovrov N G, Turpaev T M
Zh Evol Biokhim Fiziol. 1993 Jul-Aug;29(4):371-8.
In experiments on chick embryos from various age groups, studies have been made of the effect of a blockader of serotonin receptors--cyproheptadine--and blockader of adrenoreceptors--propranolol--on rhythmic activity of the amnion in vivo after injection of the substances into amniotic fluid. Stimulating effect of propranolol from the 9th to the 19th day of incubation and inhibitory effect of cyproheptadine from the 5th to the 19th day of incubation were revealed. These results indicate the existence of serotonin and noradrenaline in the amniotic fluid of chick embryo. High performance liquid chromatography directly confirmed this conclusion.
在对不同年龄组鸡胚进行的实验中，研究了血清素受体阻滞剂——赛庚啶和肾上腺素能受体阻滞剂——普萘洛尔，在将这些物质注入羊水后对羊膜在体内的节律性活动的影响。结果显示，在孵化的第9天至第19天，普萘洛尔具有刺激作用；在孵化的第5天至第19天，赛庚啶具有抑制作用。这些结果表明鸡胚羊水内存在血清素和去甲肾上腺素。高效液相色谱法直接证实了这一结论。
