Teramoto N, Tonoyama Y, Akagi T, Sarker A B, Yoshino T, Yamadori I, Takahashi K
Second Department of Pathology, Okayama University Medical School, Japan.
Acta Med Okayama. 1994 Aug;48(4):189-93. doi: 10.18926/AMO/31091.
The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells.
对单细胞聚合酶链反应（PCR）的敏感性和特异性进行了研究。其高敏感性能够检测单拷贝基因，如石蜡切片中的人I型嗜T细胞病毒基因组。用这种方法获得阳性信号的比率受靶细胞中基因拷贝数的影响。如果正确且仔细地遵循该程序，特异性是令人满意的。由于单细胞PCR是一种耗时的方法，需要技巧和经验来准确挑选靶细胞，因此该方法的适用性有限。当用于分析组织学鉴定细胞中的单个或少数拷贝基因时，它的效果最佳。
