a PhD Student, Department of Oral Anatomy and Developmental Biology, Division of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea.
Angle Orthod. 2012 Jan;82(1):107-14. doi: 10.2319/030911-173.1. Epub 2011 Aug 1.
To evaluate the localization of osteopontin (OPN) and osterix in periodontal tissue during experimental tooth movement with heavy force in rats.
Nickel-titanium closed-coil springs were used to create a 100 g mesial force to the maxillary first molars. On days 3, 7, 10, and 14 after force application, histological changes in periodontium were examined by immunohistochemistry using proliferating cell nuclear antigen (PCNA), OPN, and osterix.
PCNA-positive cells were found close to the alveolar bone and cementum on both sides. OPN-positive cells were observed along the cementing line of the cementum and bone on both sides and also were visible along with newly formed fibers in the periodontal ligament on the tension side. Osterix-positive cells were strongly detected on the surface of the alveolar bone and cementum on both sides.
During tooth movement, periodontal remodeling occurs on both sides. These results indicate that OPN and osterix may play an important role of differentiation and osteoblasts and cementoblasts matrix formation during periodontal tissue remodeling.
评价在大鼠正畸牙齿移动实验中，骨桥蛋白（OPN）和骨钙素（osterix）在牙周组织中的定位。
使用镍钛闭合线圈弹簧对大鼠上颌第一磨牙施加 100g 的近中向力。在力应用后第 3、7、10 和 14 天，通过免疫组织化学法使用增殖细胞核抗原（PCNA）、OPN 和骨钙素检查牙周组织的组织学变化。
PCNA 阳性细胞位于牙槽骨和牙骨质的两侧。OPN 阳性细胞沿牙骨质和骨的粘合线两侧观察到，在张力侧牙周韧带中也可见新形成的纤维。骨钙素阳性细胞在牙槽骨和牙骨质的两侧表面强烈检测到。
在牙齿移动过程中，两侧均发生牙周重塑。这些结果表明，OPN 和骨钙素可能在牙周组织重塑过程中对成骨细胞和牙骨质细胞的分化以及基质形成起着重要作用。
