Ideguchi H, Ishikawa A, Futata Y, Yamada Y, Ono Y, Sugisaki S, Hamasaki N
Department of Clinical Chemistry and Laboratory Medicine, Fukuoka University Hospital, Japan.
Ann Clin Lab Sci. 1994 Sep-Oct;24(5):412-21.
A comprehensive scheme for the systematic investigation of hemolytic anemia is proposed. In addition to routinely available tests for the diagnosis of hemolytic anemia, investigation of hemoglobin, analysis of red blood cell membrane proteins, and a complete panel of red blood cell enzyme measurements were included. The adoption of red blood cell enzyme measurements to routine use was facilitated by the automation of these procedures. The resolving power of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) procedure provided a tool for the identification of abnormalities in the red blood cell membrane proteins. Such a comprehensive scheme was instrumental in systematically identifying and confirming 48 cases of hemolytic anemia which otherwise would have escaped detection during the period October 1990 to September 1993 in our institution (Fukuoka University Hospital).
本文提出了一项用于系统性调查溶血性贫血的综合方案。除了用于诊断溶血性贫血的常规可用检测外，还包括血红蛋白检测、红细胞膜蛋白分析以及一整套红细胞酶测量。这些检测程序的自动化促进了红细胞酶测量在常规检测中的应用。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）程序的分辨能力为识别红细胞膜蛋白异常提供了一种工具。这样一个综合方案有助于系统性地识别和确诊48例溶血性贫血病例，否则这些病例在1990年10月至1993年9月期间于我们机构（福冈大学医院）可能会漏诊。
