Smith B E, O'Day D H, Proteau G A
Department of Zoology, Erindale College, University of Toronto, Mississauga, Canada.
Biochem Biophys Res Commun. 1995 Jan 5;206(1):401-7. doi: 10.1006/bbrc.1995.1055.
Studies with Li+, an inhibitor of phosphoinositide metabolism, demonstrated that an early response of Saccharomyces cerevisiae to a-factor pheromone was negatively affected by this cation. This was monitored by the production of beta-galactosidase from a reporter construct containing the promoter region of the yeast STE3 gene and the coding region of the E. coli LacZ gene. Growth and progression through the cell cycle were also affected by Li+ and analysis of budded/unbudded ratios revealed that Li+ caused yeast cells to arrest in G1. These data provide support for the role of inositol phosphates in the mating response and cell cycle of Saccharomyces cerevisiae.
对磷酸肌醇代谢抑制剂Li⁺的研究表明，酿酒酵母对α-因子信息素的早期反应受到这种阳离子的负面影响。这是通过含有酵母STE3基因启动子区域和大肠杆菌LacZ基因编码区域的报告构建体产生β-半乳糖苷酶来监测的。Li⁺也影响细胞周期的生长和进程，对出芽/未出芽比例的分析表明，Li⁺导致酵母细胞在G1期停滞。这些数据为肌醇磷酸在酿酒酵母交配反应和细胞周期中的作用提供了支持。
